human serotonin transporter sert  (Revvity)

Journal: Biology of Mood & Anxiety Disorders

Article Title: Increased anxiety in corticotropin-releasing factor type 2 receptor-null mice requires recent acute stress exposure and is associated with dysregulated serotonergic activity in limbic brain areas

doi: 10.1186/2045-5380-4-1

Figure Lengend Snippet: Schematic representation of experimental protocols and timelines. (A) Separate cohorts of CRFR2-null and control mice were tested for anxiety-like behaviour in the light/dark transfer and open-field tests: under basal conditions; immediately following ARS; following CVMS and again 24 to 48 h after an ARS applied 3 weeks after the end of CVMS; 24 to 48 h following ARS. (B) CRFR2-null and control mice were exposed to no stress, ARS or CVMS, and mRNA expression of stress-related genes and serotonin transporter (SERT) binding were quantified 12 h after the end of stress. 5-HT/5HIAA content in brain nuclei were quantified in unstressed mice and 24 h after ARS. (C) LCMRglu was measured in CRFR2-null and control mice one hour after administration of saline or 5-HTR agonist. (D) CRFR2 mRNA levels in brain were quantified over a 48-h time course following ARS or 7 days after CVMS in control mice. , ARS; , CVMS; d, days; w, weeks.

Article Snippet: Serotonin transporter (SERT) binding was determined on brain sections, cut as above, using ( 3 H)-paroxetine (Perkin Elmer, UK) as previously described [ ].

Techniques: Control, Expressing, Binding Assay, Saline

Journal: Biology of Mood & Anxiety Disorders

Article Title: Increased anxiety in corticotropin-releasing factor type 2 receptor-null mice requires recent acute stress exposure and is associated with dysregulated serotonergic activity in limbic brain areas

doi: 10.1186/2045-5380-4-1

Figure Lengend Snippet: Serotonergic and corticosteroid receptor expression are regulated differentially in response to stress in CRFR2-null mice. (A) mRNA expression of 5-HT 1A R in the (B) dorsal raphe nucleus (DRN) and (C) median raphe nucleus (MRN); (D) tryptophan hydroxylase 2 (TPH2) mRNA in (E) DRN and (F) MRN; (G) serotonin transporter (SERT) protein expression in (H) hippocampal CA1, (I) CA2 and (J,K) subiculum (S); (L) glucocorticoid receptor (GR) mRNA expression in (M) CA1, (N) CA2 and (O,P) the paraventricular nucleus (PVN). Data presented as mean ± SEM for mRNA levels or densitometry signal (SERT). N = 6 to 8. ANOVA critical F (2,36) value = 3.259 for P ≤ 0.05, 5.248 for P ≤ 0.01, 8.420 for P ≤ 0.001. # P < 0.05, ## P < 0.01, ### P < 0.001 for effect of stress across genotypes. * P < 0.05, ** P < 0.01, *** P < 0.001 ~ P <0.10 in post-hoc analysis. ARS increased CRFR2 mRNA expression in whole brain of mice at all times points up to 48 h post-stress (Q) , whereas CVMS decreased CRFR2 expression 7 days after the end of the CVMS protocol (R) . Data presented as mean ± SEM. N = 8. ANOVA critical F (5,42) value = 2.438 for P ≤ 0.05, 3.488 for P ≤ 0.01. * P < 0.05, ** P < 0.01 as compared with 0 hours in post-hoc analysis or with control group.

Article Snippet: Serotonin transporter (SERT) binding was determined on brain sections, cut as above, using ( 3 H)-paroxetine (Perkin Elmer, UK) as previously described [ ].

Techniques: Expressing, Control